![]() In single-cell migration, ECM confinement, such as the architecture or pore size of ECM, determines whether the cells use a mesenchymal or amoeboid migration pattern. While biochemical signaling networks regulate the motility of migrating cells, increasing evidence shows that biophysical cues from the extracellular matrix (ECM) play an essential role in driving the transition of migration patterns. During migration, cells apply versatile strategies to adjust for widely diverse microenvironments and interchange their migration patterns accordingly. Overall, a high-vorticity collective motion at the migration front promoted by parallel topography suggests a cause of the transition of collective to disseminated cell migration.Ĭell migration is critical for physiological and pathological processes such as embryogenesis, wound healing, cancer invasion, and metastasis. In addition, elongated cell morphology and frequent protrusions induced by topography may further contribute to the collective vortex motion. Enhanced collective vortex motion colocalizes with cell monolayer defects where cells extend protrusions into the free space, suggesting that topography-driven cell crawling for defect closure promotes the collective vortex motion. Furthermore, high vorticity but not velocity is correlated with disseminated cell numbers on parallel topography. Notably, a fluid-like collective motion with high vorticity is enhanced at the migration front on parallel topography. After 120 hour migration, MCF7-GFP-H2B-mCherry breast cancer cells display more disseminated cells at the migration front on parallel topography than on other topographies. This study applies a collective cell migration model with and without 800 nm wide aligned nanogrooves parallel, perpendicular, or diagonal to the cell migration direction. However, it remains unclear how anisotropic topography promotes the transition of collective to disseminated cell migration. JupyterLab – h ttps://anaconda.Cells detached and disseminated away from collectively migrating cells are frequently found during tumor invasion at the invasion front, where extracellular matrix (ECM) fibers are parallel to the cell migration direction.My Zsh: framework for managing your Zsh configuration –.Terminal for scientific computing (git, bash and more), and Scientific pipelines Cuda/Tensorflow combination according to GPU specs –.Segmentation with StarDist and SplineDist Git – apt install git (intallation instruction on the Linux Terminal).CellProfiler – General CellProfiler Linux build instructions:.ilastik 1.4.0b27 or newer – gpu-enabled build –.These were the tools used for executing the practicals in this course: BioImage Model Zoo ![]() The practicals in this course were run on virtual machines with Linux Ubuntu 18.04 Operating Systems. BioImage Model Zoo – lecture and questions.Image data resource – lecture and questions.Depositing and accessing data at EMPIAR – practical.EMPIAR: past, present and future – lecture and questions.EMPIAR, the Electron Microscopy Public Image Archive.Terminal for scientific computing (git, bash and more) – Practical.Segmentation with StarDist and SplineDist – lecture and questions.
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